Fig. 6.

Mutation of PGC-1α at Thr295 reverses the inhibitory effects of shikonin on CAF-stimulated metastasis in TNBC cells in vivo. (A) HE staining of lung section (scale bars: 2 mm; 200 μm). (B) Quantification of lung metastasis. Number of nodules and average area of metastatic foci per lung section (n = 10). (C) Representative images of lung sections with immunofluorescent staining of TOM20 expression (magnification, ×400; scale bars, 25 μm). Five tumor nodules were selected in a lung section, and lung sections from ten different lung sections were used (n = 50). The ratio of mitochondrial area to nuclear area was calculated using ImageJ software. (D) Representative images of lung sections immunostained for PGC-1α, ERRα, and COX IV (magnification, ×400; scale bars, 25 μm). The intensities of PGC-1α, ERRα, and COX IV per lung section were calculated using ImageJ software from ten different lung sections (n = 10). Data are presented as mean ± SD. ^**p < 0.01, ns: not significant