FIG. 3.
Immunolocalization of mutant hCAR constructs in polarized MDCK epithelial cells. Polarized MDCK cells transfected with wild-type hCAR, hCARtls, or hCARgpi or with expression vector alone (Neo) were cultured on permeable supports and then fixed, permeabilized, stained with anti-CAR, and examined by confocal microscopy as described in Materials and Methods. No fluorescent staining was seen in controls with nonimmune IgG. Magnification, ×17.6 (XY) and ×35.2 (XZ) (original magnification, ×20 [XY] and ×40 [XZ]).