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. Author manuscript; available in PMC: 2024 Jun 27.
Published in final edited form as: Addict Neurosci. 2024 May 9;11:100158. doi: 10.1016/j.addicn.2024.100158

Fig. 4. Sex or estrous cycle had no effect on incubation of methamphetamine craving.

Fig. 4.

(A-B) Seeking test on abstinence days 1 and 28 in male and female rats. a) Data are mean ± SEM of lever presses on the previously active and inactive lever during the 30-min seeking test on abstinence day 1. b) Data are mean ± SEM of lever presses on the previously active and inactive lever during the 2-h seeking test on abstinence day 28. c) Left panel: Data are mean ± SEM of lever presses on the previously active and inactive lever during the 30-min seeking test on abstinence day 1 and the first 30 min of the 2-h seeking test on abstinence day 28. Right panel: Data are mean ± SEM of calculated incubation slopes using data from the 30-min seeking test on abstinence day 1 and the first 30 min of the seeking test on abstinence day 28. Incubation slope = active lever presses on abstinence day 28 – active lever presses on abstinence day 1/(28–1). Green circles represent data for the individual rat. Magenta circles represent individual data from female rats in the proestrus stage; red circles represent individual data from female rats in the estrus stage; orange circles represent individual data from female rats in the metestrus stage; blue circles represent individual data from female rats in the diestrus stage. *Main effect of Abstinence Day, p < 0.05. n (male) = 7 and n (female) = 17. Abstinence day 1: n (non-estrus) = 10 and n (estrus) = 7. Abstinence day 28: n (non-estrus) = 11 and n (estrus) = 6.