Kinetics of changes in the G1:G2/M ratio, in the number of annexin V-positive cells, and in caspase-3 activity in HeLa cells that expressed C81. HeLa cells were transfected with pME18Neo that encoded Flag-tagged C81 or the control pME18Neo-Flag together with (A and B) or without (C) the GFP expression vector pEGFP-N1. The G1:G2/M ratio (A), the number of annexin V-positive cells (B), and caspase-3 activity (C) were determined, as described in the legend to Fig. 2, 12, 24, 36, 48, 60 and 72 h after transfection.