Extended Data Figure 5: TXNRD1 inhibition during senescence induction suppresses the SASP.

a, Schematic of experimental design for determining the effects of TXNRD1 inhibition during induction of senescence in IMR90 cells.

b, Heatmap of the SASP genes that were significantly suppressed by both TXNRD1 knockdown and Tri-1 treatment based on RNA-seq analysis. The relative expression levels per replicate and average fold change differences are shown (n = 3 biologically independent experiments).

c,d, Expression of the indicated SASP genes in control and oncogenic RAS-induced senescent IMR90 cells with or without TXNRD1 knockdown (c) or Tri-1 treatment (d) was determined by RT-qPCR. n = 4 biologically independent experiments.

e,f, Expression of the indicated proteins in oncogenic RAS-induced senescent IMR90 cells with or without TXNRD1 knockout was determined by immunoblot (e), and expression of the indicated SASP genes was determined by RT-qPCR (f).

Data represent mean ± s.e.m. n = 3 biologically independent experiments unless otherwise stated. P-values were calculated using a two-tailed t test.