FIG. 5.

LMB does not relocate EB2Cter to the cell nucleus. HeLa cells were transfected with plasmids expressing Flag.Rev and Flag.EB2Cter. Twenty-four hours after transfection, cells were treated for 3 h with 10 nM (b and e) or 25 nM (c and f) LMB or not treated (a and d). Flag.Rev and Flag.EB2Cter localization was performed by immunofluorescence with an anti-Flag monoclonal antibody. Whereas Rev completely relocates to the cell nucleus upon LMB treatment (b and c), EB2Cter localization is not affected by high concentrations of LMB (e and f).