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. 2024 Jul;30(7):1442–1446. doi: 10.3201/eid3007.231040

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Emerging Infectious Diseases is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.

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Phylogenetic tree (left) and aligned sequences (right) of Orientia spp. and locally endemic Rickettsia spp. in a study of Orientia genus endemicity among severe infectious disease cohorts, Uganda. We compared the 16S rRNA gene with an Orientia infection (case D) in Uganda. We aligned the 96-bp amplicon region and created the tree by using the neighbor-joining algorithm in R (The R Foundation for Statistical Computing, https://www.r-project.org). GenBank accession numbers of reference sequences are in parentheses. A single polymorphism aligned with Candidatus O. chuto, possibly differentiating case D from other Orientia spp. Scale bar indicates nucleotide substitutions per site.