Table 2. Comparison of real-time PCR results for case-patient 1 and retrospective SFG rickettsiosis case-patients in study of newly recognized SFG Rickettsia as cause of severe RMSF-like illness, northern California, USA*.
| SFG rickettsiosis case-patient | Specimen type | Cycle threshold value, by test type |
||||
|---|---|---|---|---|---|---|
| Triplex Rickettsia spp. | Triplex R. rickettsii SNP | Triplex R. typhi SNP | RRi6 R. rickettsii | Rickettsia sp. CA6269 ompA | ||
| 1 | Plasma | 24.9 | ND | ND | 30.5 | 29.6 |
| 2 | Serum | 34.3 | ND | ND | 34.3 | 34.4 |
| 3 | Plasma | 23.3 | 25.2 | ND | 25.4 | ND |
| 4 | Serum | 30.3 | 27 | ND | 34 | ND |
| 5 | Serum | 32.8 | 30.2 | ND | 35 | ND |
| 6 | Serum | 32.2 | 32.8 | ND | 33.6 | ND |
| 7 | Plasma | 29.5 | 29.2 | ND | 33.3 | ND |
| 8 | Serum | 20.8 | 19.2 | ND | 25.1 | ND |
| 9 | Plasma | 28.6 | 27.3 | ND | 30.2 | ND |
*ND, not detected; RMSF, Rocky Mountain spotted fever; SFG, spotted fever group; SNP, single-nucleotide polymorphism.