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. Author manuscript; available in PMC: 2025 Jun 1.
Published in final edited form as: Curr Protoc. 2024 Jun;4(6):e1073. doi: 10.1002/cpz1.1073

Figure 2: Suction Blister Biopsy Procedure and Formation.

Figure 2:

Visual time course of blister formation. 0 minutes of negative pressure (A). 10 minutes of negative pressure (B). 15 minutes of negative pressure, early signs of vesicle formation in the center and bottom right orifices. Yellow arrows point to vesicle formation (C). 20 minutes of negative pressure, blister vesicle formation and enlargement in 3 orifices, as indicated by yellow arrows (D). 35 minutes of negative pressure, vesicle formation observed in all 5 orifices (E). 45 minutes of negative pressure, top left blister beginning to leak, shown by yellow arrow, but other sites not yet fully expanded to 10 mm. Yellow bars indicate space between blister and plate (F). 55 minutes of negative pressure, top left blister continuing to leak, all other sites fully expanded (G). 5 fully formed blisters immediately after cup removal at 55 minutes (H).