TABLE 1.
Reduction of input infectivity (V/V0) by coincubation with murine sera obtained after a single subcutaneous immunization with DB and sfDB
| DB dose (μg) | Animal no. | Serum dilutiona
|
sfDB dose (μg) | Animal no. | Serum dilutiona
|
|||
|---|---|---|---|---|---|---|---|---|
| V/V0 = 1 | V/V0 = 0.5 | V/V0 = 1 | V/V0 = 0.5 | |||||
| 20 | 1–3 (p)b | 1:96 | 1:768 | 20 | 1–3 (p) | 1:24 | 1:192 | |
| 4 | 1:48 | 1:384 | 4 | 1:12 | 1:192 | |||
| 5 | 1:48 | 1:384 | 5 | 1:24 | 1:192 | |||
| 6 | 1:48 | 1:384 | 6 | 1:24 | 1:192 | |||
| 2 | 1–3 (p) | 1:96 | 1:768 | 2 | 1–3 (p) | ND | ND | |
| 4 | 1:48 | 1:384 | 4 | 1:6 | 1:96 | |||
| 5 | 1:48 | 1:384 | 5 | 1:6 | 1:96 | |||
| 6 | 1:48 | 1:384 | 6 | 0 | 1:24 | |||
| 0.2 | 1 | 1:24 | 1:192 | 0.2 | 1 | 0 | 1:24 | |
| 2 | 1:12 | 1:192 | 2 | 0 | 1:12 | |||
| 3 | 1:12 | 1:192 | 3 | 0 | 1:6 | |||
| 0.02 | 1 | 0 | 1:96 | 0.02 | 1 | 1:6 | 1:24 | |
| 2 | 1:12 | 1:96 | 2 | 0 | 1:6 | |||
| 3 | 1:12 | 1:96 | 3 | 0 | 0 | |||
a
V/V0 = 1, last serum dilution that resulted in complete reduction of input infectivity; V/V0 = 0.5, last serum dilution that resulted in ≥50% neutralization of input infectivity. ND, not determined.
b
Sera from three animals (1 to 3) were pooled prior to analysis.