TABLE 2.
Anti-HCMV-specific IgG antibody isotype profile of sera from mice immunized intraperitoneally three times at 4-week intervals with 20 μg of DB
| Animal no. | Time after initial injection (wk) | Endpoint dilutiona
|
Ratio, IgG1/IgG2a | |
|---|---|---|---|---|
| IgG1 | IgG2a | |||
| 1–3 (p)b | 12 | 352 (±48) | 1,639 (±210) | 0.21 |
| 4 | 32 | 223 (±77) | 1,277 (±123) | 0.17 |
| 5 | 32 | 362 (±38) | 1,365 (±150) | 0.26 |
a
Endpoint dilution of serum that resulted in more than 2.5 times the absorption of a null serum for IgG1-specific or IgG2a-specific anti-HCMV antibodies. Values in parentheses are the maximal deviations from the means for three individually performed experiments.
b
Sera from three animals (1 to 3) were pooled prior to analysis.