FIG. 6.

KD1 and KD3 reduce the growth of human tumors in nude mice. In all experiments, 10⁷ cells were injected into each hind flank of nude mice. (a) A549 cells were mock infected or infected with KD3 (10 PFU/cell). After 3 h, the cells were trypsinized and added to trypsinized uninfected A549 cells such that 1 of 10,000 cells was infected. Cells were injected, and tumor growth was measured over 5 weeks. P = 0.007. (b) A549 cells were injected. When the tumors reached about 50 to 70 μl, they were injected with DMEM (mock) or KD3 (5 × 10⁸ PFU). The fold increase in tumor size was measured over 5 weeks. P = 0.048. (c) Same as in panel b except that tumors were measured and then injected with 5 × 10⁷ PFU of the indicated viruses on day 0 and at weeks 1, 2, and 3. For the following comparisons P was as indicated: KD3 and mock, 0.015; KD1 and mock, 0.015; 309 and mock, 0.014; dl01/07 and mock, 0.107; KD3 and dl01/07, 0.010; KD3 and KD1, 0.473; KD3 and 309, 0.461. (d) Hep 3B cells were injected. When tumors reached about 100 μl, they were injected with 5 × 10⁷ PFU of the indicated viruses on day 0 and twice per week until 3 weeks. For the following comparisons, P was as indicated: KD3 and mock, 0.030; KD1 and mock, 0.017; 309 and mock, 0.002; KD3 and KD1, 0.282; KD3 and 309, 0.003; KD1 and 309, 0.029.