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. 2024 Jun 27;15:5342. doi: 10.1038/s41467-024-49564-8

Fig. 4. CCK robustly increased both [Ca2+]i, which causes the hormonal release, and mRNA expression of fshb.

Fig. 4

a Ca2+ imaging of FSH cells applied with 1 μM CCK-8s. The shaded section indicates the time and duration of the perfusion of CCK-8s. b Dose-response curve of [Ca2+]i in FSH cells applied with CCK-8s of various concentrations (n = 4 fish;***p = 1.4e-6, 1.4e-6, 2.2e-4). c Ca2+ imaging of LH cells applied with 1 μM CCK-8s. The shaded section indicates the time and duration of the perfusion of CCK-8s. d Repetitive Ca2+ imaging trials on LH cells (n = 4 fish). eg qRT-PCR of the pituitary after incubating with CCK-8s for 48 h (e, fshb; f, lhb; g, tshb; n = 5 fish; ***p = 2.9e-6, 4.8e-5). hj qRT-PCR of the pituitary of cck2rb+/− and cck2rb−/− after incubating in CCK-8s (h, fshb; i, lhb; j, tshb; n = 5 fish; **, p = 3.7e-3). The data are mean ± SEM. ***p < 0.001, **p < 0.01, N.S. not significant, two-sided Dunnett’s test (b, eg), two-sided Student’s t test (d, hj). Source data are provided as a Source Data file.