Table 6.

Difference between the antibacterial effect of NE and NE-SSC on L. monocytogenes and E. coli O157:H7 in milk stored at 4 and 10°C.

Bacteria	Temperature	Day	0%			3%			7%			10%
			NE	NE-SCC		NE	NE-SCC		NE	NE-SCC		NE	NE-SCC
					p-value			p-value			p-value			p-value
L. monocytogenes	4°C	0	4.41 ± 0.06d	4.41 ± 0.06d	1	4.41 ± 0.06d	4.41 ± 0.06d	1	4.41 ± 0.06d	4.41 ± 0.06d	1	4.41 ± 0.06d	4.41 ± 0.06d	1
		1	5.50 ± 0.06c	5.50 ± 0.06c	1	5.48 ± 0.04c	5.39 ± 0.02c	0.134	5.16 ± 0.08c	5.03 ± 0.16c	0.503	4.92 ± 0.04c	4.63 ± 0.02c	0.003**
		3	7.53 ± 0.05b	7.53 ± 0.05b	1	7.38 ± 0.07b	7.02 ± 0.06b	0.017*	6.51 ± 0.03b	5.59 ± 0.03b	< 0.001***	5.45 ± 0.08b	5.00 ± 0.04b	0.005**
		7	8.09 ± 0.04a	8.09 ± 0.04a	1	8.06 ± 0.03a	8.13 ± 0.07a	0.437	7.52 ± 0.18a	7.14 ± 0.10a	0.135	6.88 ± 0.10a	5.46 ± 0.09a	< 0.001***
	p-value		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	< 0.001***
	10°C	0	4.41 ± 0.06c	4.41 ± 0.06c	1	4.41 ± 0.06d	4.41 ± 0.06d	1	4.41 ± 0.06d	4.41 ± 0.06d	1	4.41 ± 0.06d	4.41 ± 0.06d	1
		1	6.07 ± 0.11b	6.07 ± 0.11b	1	5.58 ± 0.07c	5.26 ± 0.05c	0.021*	5.30 ± 0.02c	4.68 ± 0.01c	< 0.001***	5.04 ± 0.03c	4.58 ± 0.06c	0.001*
		3	7.79 ± 0.12a	7.79 ± 0.12a	1	7.33 ± 0.12b	7.12 ± 0.08b	0.213	6.43 ± 0.08b	5.56 ± 0.14b	0.005**	5.90 ± 0.20b	5.11 ± 0.05b	0.019*
		7	8.04 ± 0.02a	8.04 ± 0.02a	1	8.24 ± 0.05a	8.21 ± 0.11a	0.828	7.92 ± 0.05a	7.61 ± 0.07a	0.018*	7.46 ± 0.03a	6.23 ± 0.05a	< 0.001***
	p-value		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	< 0.001***
E. coli O157:H7	4°C	0	3.99 ± 0.02d	3.99 ± 0.02d	1	3.99 ± 0.02d	3.99 ± 0.02d	1	3.99 ± 0.02b	3.99 ± 0.02b	1	3.99 ± 0.02c	3.99 ± 0.02	1
		1	4.37 ± 0.05c	4.37 ± 0.05c	1	4.44 ± 0.12c	4.34 ± 0.11c	0.534	4.15 ± 0.16b	4.15 ± 0.17b	0.979	4.10 ± 0.06bc	3.89 ± 0.22	0.399
		3	6.02 ± 0.05b	6.02 ± 0.05b	1	5.69 ± 0.11b	5.60 ± 0.00b	0.461	4.87 ± 0.08ab	4.76 ± 0.03a	0.258	4.48 ± 0.10a	4.14 ± 0.04	0.034*
		7	7.66 ± 0.03a	7.66 ± 0.03a	1	8.09 ± 0.21a	7.58 ± 0.04a	0.134	5.47 ± 0.54a	5.24 ± 0.26a	0.722	4.25 ± 0.01b	3.72 ± 0.07	0.002**
	p-value		< 0.001***	< 0.001***		< 0.001***	< 0.001***		0.021*	0.002**		0.002**	0.152
	10°C	0	3.99 ± 0.02d	3.99 ± 0.02d	1	3.99 ± 0.02d	3.99 ± 0.02c	1	3.99 ± 0.02d	3.99 ± 0.02d	1	3.99 ± 0.02c	3.99 ± 0.02b	1
		1	5.11 ± 0.06c	5.11 ± 0.06c	1	4.73 ± 0.04c	3.92 ± 0.26c	0.035*	4.33 ± 0.03c	3.57 ± 0.16c	0.009**	4.12 ± 0.07c	3.99 ± 0.05b	0.212
		3	7.05 ± 0.06b	7.05 ± 0.06b	1	6.95 ± 0.03b	6.67 ± 0.05b	0.010*	5.85 ± 0.00b	4.87 ± 0.03b	< 0.001***	4.45 ± 0.03b	4.27 ± 0.05a	0.042*
		7	8.87 ± 0.02a	8.87 ± 0.02a	1	8.54 ± 0.11a	8.23 ± 0.01a	0.055	8.01 ± 0.04a	5.20 ± 0.10a	< 0.001***	5.11 ± 0.06a	4.14 ± 0.07ab	< 0.001***
	p-value		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	< 0.001***		< 0.001***	0.010*

Values are means (log10 CFU/ml) of three experiments ± SEM.

Means within the column not sharing a similar superscript differ significantly (p < 0.05).

Column-wise comparisons were performed using ANOVA with DMRT post-hoc.

Row-wise comparisons were made using independent samples t-test.