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. 2024 Jun 27;15:5441. doi: 10.1038/s41467-024-49854-1

Fig. 7. Podocyte-specific overexpression of OTUD5 alleviates podocyte injury and DKD in T2DM mice.

Fig. 7

a A schematic diagram illustrating the procedure of T2DM-induced Otud5fl/fl mice injected with AAV-EV or AAV-OTUD5. b The levels of blood glucose. Data are presented as mean ± SD. c Ubiquitinated TAK1 was detected by immunoblotting using anti-ubiquitin antibodies in the kidneys of indicated groups. (n = 6 samples). d Representative western blot images showing phosphorylated and total protein levels of TAK1 in the kidneys of indicated groups. (n = 6 samples). The levels of serum creatinine (e), urea nitrogen (f), and urine albumin to creatinine ratio (g). h Representative H&E and PAS staining of kidney sections. (n =6 samples). i–l Representative TEM images of kidney sections and corresponding quantitative analysis. Scale bar: 1 μm. m Representative IF images of Nephrin expression. Scale bar, 20 μm. (n = 6 samples). n = 6 for each group. For e–g and j–l, P values were determined by a two-tailed unpaired t-test, and data are presented as mean ± SD.