Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Jun 28;15:5470. doi: 10.1038/s41467-024-49721-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a Chromatin immunoprecipitation (ChIP) analysis of the BrJMJ18 level across BrFLC3 in BrJMJ18^Par-carrying Par and BrJMJ18^PC-carrying DG plants. The 5^th−7^th young and healthy rosette leaves of five-week-old plants were used for the analysis. Rabbit IgG was used as a control. GADPH was used as a BrJMJ18-independent control. Control is a locus gene desert region where BrJMJ18 does not bind. The values are the mean ± standard deviation from three biological replicates. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t-test (**0.01 < p < 0.05, ***p < 0.001). DG, p = 0.0016; Par, p = 0.00027. b Expression patterns of BrFLC1, 2, 3 and 5 in Par before and after bolting under natural field conditions. The 5^th−7^th young and healthy rosette leaves were used for qPCR test. The values are the mean ± standard deviation from three biological replicates. The Par bolted at 25DAP. DAP, days after planting (14-day Par seedlings grown in cultivation pots in greenhouse were transplanted into natural field). c BrFLC3 expression patterns in BrJMJ18^PC/Par- overexpressing plants. The 5^th−7^th young and healthy rosette leaves of Par plants were used for qPCR test. The values are the mean ± standard deviation from three biological replicates. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t test (*0.01 < p < 0.05). BrJMJ18^PC-OX, p = 0.0032; BrJMJ18^Par-OX, p = 0.0023. d ChIP analysis of H3K36me3 enrichment on the BrFLC3 locus in BrJMJ18 overexpression Par lines. The values are the mean ± standard deviation from three biological replicates. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t test (** 0.001 < p < 0.01). Par, p = 0.012; BrJMJ18^Par-OX, p = 0.034. e The H3K36me3 level at BrFLC3 in DG (BrJMJ18^PC-carrying) and Par (BrJMJ18^Par-carrying). The values are the mean ± standard deviation from three biological replicates. Asterisks indicate significant differences between NC and HT, two-tailed Student’s t-test (*p < 0.05, **0.001 <p < 0.01,). DG, p = 0.021; Par, p = 0.0016. Source data are provided as a Source Data file.