Skip to main content
. 2024 Mar 5;5(6):100858. doi: 10.1016/j.xplc.2024.100858

Figure 2.

Figure 2

Domain structure and subcellular localization of GhCTSF1.

(A) Schematic diagram of the functional domains of GhCTSF1. cTP, chloroplast transit peptide predicted by TargetP.

(B) Quantitative RT–PCR (RT–qPCR) analysis of relative GhCTSF1 transcript levels in various tissues and fibers at different stages. The cotton UBQ7 gene was used as an internal control. Values are means ± SD (n = 3).

(C) Subcellular localization of the GhCTSF1–GFP fusion protein in N. benthamiana leaf epidermal cells. GhCTSF1–GFP exhibits a chloroplast localization pattern, whereas free GFP is cytosolic and nuclear. GFP, green fluorescent protein; Chlorophyll, chlorophyll autofluorescence.

(D) The GhCTSF1–mCherry construct co-localized with WTF1–GFP. Protoplasts were obtained from N. benthamiana leaves expressing GhCTSF1–mCherry and WTF1–GFP/PEND–GFP/PGL34–GFP/PIC1–GFP fusion proteins. Scale bar, 10 μm.