Figure 3.

Subcellular localization and functional complementation assays with CitCYP97B.

(A) Phylogeny of CYP97-family amino acid sequences. Abbreviations and amino acid sequence accession numbers used to construct the neighbor-joining tree are listed in Supplemental Table 5.

(B) Subcellular localization of a CitCYP97B-eGFP fusion protein in N. benthamiana leaves. PT-mCherry is a plastid marker protein.

(C) Analysis of carotenoids in β-carotene-accumulating E. coli strains. E. coli BL21 (DE3) cells harboring pACCARΔ16crtX were co-transformed with (a) pRSFDuet-CitCYP97B, (b) pRSFDuet-CitBCH2, (c) pRSFDuet-CitBCH2-CitCYP97B, or (d) pRSFDuet-CitBCH1 (left). Mass spectrometry data and structures for the carotenoids are shown at right.