FIG. 4.

Ii interacts with Vpx in HIV-2-infected cells. (a) CEMx174 cells were infected with MX or ES virus. Cell lysates were immunoprecipitated (IP) with PIN.1 or polyclonal antiserum (Ab), followed by SDS-PAGE and immunoblotting with Vpx antiserum. (b) T2 cells were infected with MX or ES virus, and immunoprecipitated with polyclonal Ii or Vpx antiserum, and subjected to SDS-PAGE and immunoblotting with polyclonal Vpx antiserum. (c) T2 cells, chronically infected with ES or MX, were immunoprecipitated with polyclonal Ii antiserum. Immunoprecipitated proteins were subjected to SDS-PAGE followed by immunoblotting using PIN.1 antiserum.