Figure 1.
Mitogen‐activated protein kinase 4 (MAPK4) expression during the development of non‐small cell lung cancer (NSCLC). (a) Schematic diagram showing subcutaneous injection of Lewis lung carcinoma (LLC) cells (5 × 105) into the right flanks of wild‐type (WT) mice. (b) Tumor volume. (c) Tumor weight. (d) Tumor pathology was analyzed by hematoxylin‐eosin (H&E) staining. (e) The expression levels of MAPK4 in tumors was analyzed by immunofluorescence and (f) quantified. (g) The colocalization of CD34 and MAPK4 in tumors were analyzed by immunofluorescence. (h) Colocation coefficient between CD34 and MAPK4 expression were quantified by ImageJ. Representative data from three independent experiments were shown. *p < 0.05, **p < 0.01. NS, no significance.