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. 2024 Apr 16;3(3):e117. doi: 10.1002/cai2.117

Figure 4.

Figure 4

Mitogen‐activated protein kinase 4 (MAPK4) silencing inhibits endothelial cell (EC) growth. Human umbilical vein ECs (HUVECs) were cultured in the supernatant of human non‐small cell lung cancer (NSCLC) cells and transiently transfected with MAPK4 small interfering RNA (siRNA) (50 nM) in 24‐well plates via Lipofectamine 3000 reagent in vitro. (a) The expression of MAPK4 in HUVECs was detected by immunofluorescence. (b) The number of cells was determined by cell counting. (c) Cell counting kit‐8 (CCK‐8) assay was used to evaluate the proliferation ability of HUVECs. (d) The tube‐forming ability of HUVECs was evaluated by tube formation assay. (e) The expression of ki‐67 in HUVECS was detected by fluorescence‐activated cell sorting. (f, g) Extracellular regulated protein kinases 1/2 (ERK1/2) and phosphorylated ERK1/2 (p‐ERK1/2) in HUVECs was detected by western blot analysis. (h, i) The expression of p‐ERK1/2 in HUVECs was detected by immunofluorescence analysis and calculated. Representative data from three independent experiments are shown. **p < 0.01, NS, no significance.