TABLE 1.

Alignment (5′ to 3′) of TRS, spacer, and RBS motifs in viral ITR DNA and in human AAVS1 DNA

DNA	TRS	Spacer	RBS
AAV2 ITRa	AGTTGG	CCACTCCCTCTCT	GCGC GCTC GCTC GCTC
Human AAVS1b	GGTTGG	GGCTCGGC	GCTC GCTC GCTC GCTG

^aThe minimum AAV2 TRS sequence for efficient Rep cleavage has recently been described by Brister and Muzyczka (3) as the 7-base core 5′-GTTGGCC-3′. Hence, the 5′ CC dinucleotide of the spacer shown may be part of the TRS motif. 

^bData are from references 26, 25, and 18.