TABLE 3.

Deletion of the spacer sequence or alteration of the distance of the spacer from the TRS and RBS motifs abrogates episomal integration^a

Episome carried by cell line	Sequence between TRS and RBS in p220.2 inserts	AAV-positive colonies (integration)
		No./total	%
102	TRS-    GG CTC GGC-RBS	56/40,000	0.140
201	TRS-              -RBS	4/90,500	0.004
202	TRS-50n+GG CTC GGC-RBS	2/74,700	0.003
203	TRS-GG CTC GGC+50n-RBS	1/69,240	0.001

^aIn episome 102, the spacer sequence is that of AAVS1; in episome 201, the spacer is deleted; in episome 202, the spacer sequence is separated from the TRS by 50 random nucleotides (50n); and in episome 203, the spacer sequence is separated from the RBS by 50 random nucleotides. Remaining details are as described in Table 2, footnote a.