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[Preprint]. 2024 Jun 17:2024.06.16.599214. [Version 1] doi: 10.1101/2024.06.16.599214

Fig. 3. When overexpressed, the phosphorylation-deficient bfmR(D58A) allele lacks complementing ability but has dominant negative effects.

Fig. 3.

Strains harbored the indicated bfmR allele in its native chromosomal locus or via plasmid. Expression from plasmid-borne bfmR was driven by 25 (A, B) or 75 (C, D) μM IPTG. A, C. Immunoblot analysis of total BfmR levels. Data show mean ± s.d. (n=3). A representative blot is shown below each graph. B, D. Antibiotic resistance assays. Data show geometric mean ± s.d., n=3 (B), n=5 (D). In D, one-way ANOVA with Dunnett’s test was used to compare bfmR-overexpressed strains to the control (WT with empty vector). ns, P>0.05; ***, P≤0.001.