Fig. 3:

CS-GAG sulfation code is generally conserved between species in the healthy brain A Comparison of the relative abundance of each CS isomer in the control mPFC, EC and vHPC of mouse (N = 10) brain measured by LC-MS/MS B Like human brain, the mouse vHPC is hypersulfated compared to both the EC and mPFC, however, in this species the mPFC and EC do not differ in average sulfation (Kruskal-Wallis test: P = 0.0001, followed by Dunn’s test) C All CS-isomers are expressed in significantly different abundances across the healthy mouse brain (ANOVA: isomer effect: F(1,11) = 13984, P < 0.0001; region effect: F(1, 12) = 0.01896, P = 0.940; isomer × region : F(2, 16) = 97.49, P < 0.0001, followed by Tukey’s test). Avg#S/ΔCS: average number of sulfates per chondroitin sulfate disaccharide, CS-GAG: chondroitin sulfate glycosaminoglycan, LC-MS/MS: liquid chromatography tandem mass spectrometry, mPFC: medial prefrontal cortex, EC: entorhinal cortex, vHPC: ventral hippocampus, ANOVA: analysis of variance.