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[Preprint]. 2024 Jun 21:2024.02.09.579711. [Version 3] doi: 10.1101/2024.02.09.579711

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Fig. 6: — Perineuronal net sulfation code and labeling pattern are preserved after the experience of early-life stress in both human and mouse brain A-C Within each human brain region CS-GAG sulfation code is unaffected by depression, suicide (DS) and child abuse (DS-CA) when compared to controls (CTRL) (ANOVA: vmPFC group × isomer: F(3,67) = 0.132, P =0.929; EC group × isomer: F(3,13) = 1.448, P = 0.275; HPC group × isomer: F(2,6) = 1.210, P = 0.372) D-F Although we previously reported a significant increase in WFL+ PNNs in the vmPFC, overall labeling pattern is conserved between groups in all brain regions examined (ANCOVA with age as covariate: vmPFC group × labeling: F(4,50) = 2.104, P = 0.094; EC group × labeling: F(3,38) = 0.471, P = 0.65; HPC group × labeling: F(3,47) = 0.152, P = 0.961) G Limited bedding and nesting from P2–9 is associated with a significant increase in WFL+ PNNS in the mPFC of adult mice (N = 9) compared to control (N =10) (Welch’s t-test: P = 0.0175) H-J Within each mouse brain region CS-GAG sulfation code is unaffected by early-life stress (ANOVA: mPFC group × isomer: F(1,21) = 1.761, P = 0.200; EC group × isomer: F(1,9) = 5.029, P = 0.464 although does not pass Bonferroni’s multiple comparisons test; vHPC group × isomer: F(1,22) = 0.683, P = 0.424) K-M Although we report an increase in WFL+ PNNs in the mPFC of mice, the overall landscape of PNN labeling by WFL and ACAN is resilient to ELS (ANOVA: mPFC group × labeling: F(2,34) = 0.248, P = 0.782; EC group × labeling: F(2,32) = 1.33, P = 0.279; vHPC group × labeling: F(2, 32) = 0.140, P = 0.870). CTRL: psychiatrically healthy control, DS: depressed suicide, DS-CA: depressed suicide with a history of child abuse, CS-GAG: chondroitin sulfate glycosaminoglycan, WFL: Wisteria Floribunda Lectin, ACAN: anti-aggrecan core protein, vmPFC: ventromedial prefrontal cortex, EC: entorhinal cortex, HPC: hippocampus, ANCOVA: analysis of covariance, mPFC: medial prefrontal cortex, EC: entorhinal cortex, vHPC: ventral hippocampus, ANOVA: analysis of variance.