Table II:
Specific labeling details
| Abbreviation | Primary | Labels | Target species | Product | Fluorophore conjugated-secondary |
|---|---|---|---|---|---|
| Human samples | |||||
| WFL/WFA | Biotinylated Wisteria Floribunda Lectin | Perineuronal nets | Human | Vector Laboratories, B-1355, 1:2500 | Alexa 488-Streptavidin, Jackson ImmunoResearch, 016-540-084, 1:500 |
| NeuN | Anti-neuronal nuclei | Neurons | Human | Abcam, ab104225, 1:1000 | DyLight-594 antirabbit, Jackson ImmunoResearch, 111-515-144, 1:500 |
| ACAN | Anti-chondroitin sulfate proteoglycan brain core protein (Cat-301) | Perineuronal nets | Human | Millipore, MAB5284, 1:500 | Alexa-647 antimouse, Jackson ImmunoResearch, 115-605-146, 1:500 |
| Mouse samples | |||||
| WFL/WFA | Biotinylated Wisteria Floribunda Agglutin | Perineuronal nets | Mouse | Vector Laboratories, B-1355, 1:1500 | Cy3-Streptavidin, Jackson ImmunoResearch, 016-160-084, 1:500 |
| NeuN | Anti-neuronal nuclei | Neurons | Mouse | Millipore, MAB377, 1:500 | Alexa-647 antimouse, Jackson ImmunoResearch, 115-605-146, 1:500 |
| ACAN | Anti-aggrecan | Perineuronal nets | Mouse | Millipore, AB 1031, 1:100 | Alexa-488 antirabbit. Jackson ImmunoResearch, 111-485-144, 1:500 |