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Ca2+ does not induce dissociation of Syt1 C2AB from membrane-anchored SNARE complex. The graph shows fluorescence spectra of nanodisc-anchored SC-59-bimane alone (black curve), or in the presence of C2AB-T285W and Mg-ATP plus 1 mM EGTA (red curve) or 1 mM Ca2+ (blue curve). Mg-ATP was included in the samples to hinder non-specific C2AB-SNARE complex interactions (47).
