Figure 1: Overview of CRISPR FACS screens and tunable parameters.

A) Schematic of pooled CRISPR FACS screens. Perturbations that differentially affect the expression of a target protein of interest are mixed together in a pool of modified cells. To infer the effect of each gRNA, cells are sorted into bins based on expression of a target protein using FACS; gRNA abundance is compared across bins through sequencing. B) Experimental design considerations focused on reducing cell requirements, including the effect of changing i. gRNA coverage, ii. MOI, and iii. FACS bin configuration.