Table 1: Summary of the experimental recording conditions used by the three labs.
The datasets are described in the following order: Anesthesia, Preparation, Electrode Placement, Sound Stimuli, Recording, Sound Intensity, and Distance of the Speaker. The specific methods employed by each lab—Manor Lab (Lab A), Marcotti Lab (Lab B), Liberman Lab (Lab C)—are detailed in the supplementary information section.
Methods | Lab A | Lab B | Lab C |
---|---|---|---|
Anesthesia | Ketamine (90 mg/kg) + Xylazine (10 mg/kg) | Ketamine (100 mg/kg) + Xylazine (10 mg/kg) | Ketamine (100 mg/kg) + Xylazine (10 mg/kg) |
Environme nt | Soundproof chamber, heating pad (37°C) | Soundproof chamber, heating pad (37°C) | Soundproof chamber, heating pad (37°C) |
Electrode Placement | Subcutaneous recording electrode at vertex, reference behind right pinna, ground on left leg | Subdermal electrodes behind pinna (reference and ground), vertex (active) | Needle electrodes: vertex to ipsilateral pinna (recording), ground near tail |
Sound Stimuli | 5-ms tone pips (0.5 ms cos2 rise-fall), 21/sec | 5-ms pips (1.0-ms rise-fall with cos2 onset envelope), 42.6/sec | 5-ms pips (0.5-ms rise-fall with cos2 onset envelope), 30/sec |
Recording | Filtered (300 Hz - 3 kHz), averaged using BioSigRZ software, 512 responses averaged | Customized software (Ingham et al., 2011), RZ6 auditory processor, 256 responses averaged | Amplified (10,000X), filtered (100 Hz - 3 kHz), averaged with A-D board in LabVIEW system, 1024 responses averaged |
Sound Intensity | Decreased from 90 dB SPL to 10/20 dB SPL in 5 dB steps | 0–95 dB SPL in 5 dB steps | Raised from ~10 dB below threshold to 80 dB SPL in 5 dB steps |
Speaker distance | Open-field - 10 cm from ear | Open-field - 10 cm from ear | Closed-field - ~3 cm from the eardrum |
Mouse age/strains used | 3-month SAMP8 (Senescence-Accelerated Mouse-Prone 8) (Takeda et al. 1981) | 1-month C57Bl/6N with and without corrected CDH23 | 7-week C57Bl/6J |