Figure 3. Various ubiquitin processors are enriched in purified non-cytosolic fractions in response to arsenite.
3A – Schematic of sample preparation for proteomics analysis of the cytosolic and non-cytosolic fractions during arsenite stress.
3B – Volcano plot of log2(fold enrichment) of various proteins in the non-cytosolic versus cytosol fractions. Highlighted proteins are localized to the cytosol, endoplasmic reticulum, mitochondria, and endosomes. Lists were generated from The Human Protein Atlas.
3C – Volcano plots showing enrichments under arsenite stress of proteins with ubiquitin binding domains in the non-cytosolic and cytosolic fractions. Known VCP adaptors were highlighted among the proteins with UBDs in both plots.
3D – Fold change under arsenite stress of VCP adaptors and other identified UBD-containing proteins within the non-cytosolic fraction.
3E – Gene ontology enrichment of proteins with ubiquitin binding domains enriched in the purified non-cytosolic fraction under arsenite stress (fold change > 1.5 and p-value < 0.05).