Extended Data Fig. 2: The SARS-CoV-2-nsp5^T169Insp12^V792I virus showed decreased sensitivity against nirmatrelvir and remdesivir therapies in vitro.

Vero E6 cells were treated with indicated concentration of nirmatrelvir at pre-infection, at infection or at post-infection period and infected with 200 TCID₅₀/well of SARS-CoV-2-nsp5^T169Insp12^V792I (A, C, E) or WT (B, D, F). After 48 hours, the cell monolayer was fixed and stained with SARS-CoV-2 NP specific monoclonal antibody (red) and counterstained with DAPI (blue). Vero E6 cells were treated with indicated concentration of remdesivir at pre-treatment, at infection or at post-infection period and infected with 200 TCID₅₀/well of SARS-CoV-2-nsp5^T169Insp12^V792I (G, I, K) or WT (H, J, L). After 48 hours, the cell monolayer was fixed and stained with SARS-CoV-2 NP specific monoclonal antibody (red) and counterstained the nucleus with DAPI (blue). Represent images from 2 (remdesivir) and 3 (nirmatrelvir) independent experiments.