Table 4.

Assembly features of Platynereis genomes

	P. dumerilii (HiFi assembly) #	P. massiliensis	P. megalops
‘Predicted’ genome size	~940 Mbp	~730 Mbp	~440 Mbp**
Assembly size	1.42 Gbp	1.43 Gbp	1.88 Gbp
Scaffold No.	163	1,478	370
N50	71 Mbp	4 Mbp	49 Mbp
L50	7	59	11
RE content	51%	58%	66%
Heterozygosity	3.2%	1.2%	10%**
BUSCO	96.0% complete (1.3% dupl.)	94.2% complete (2.5% dupl.)	94.8% complete (0.4% dupl.)

Note: The ‘predicted’ genome size and the heterozygosity are calculated based on short-read Illumina DNA sequencing using the same algorithm (Materials and Methods). The polymorphism rate can greatly impact the estimated values and they are highly correlated (Ranallo-Benavidez et al., 2020). BUSCO version 5 was used to approximate genome completion for all three species.

^*dupl is the shortened text for duplication, a measure of how many conserved single-copies appear more than once in the assembly.

^#The P. dumerilii Hi-Fi assembly is not yet annotated, and was not the assembly used for the prior analyses. This genome will be annotated in unison with the other Platynereis genome assembly, for more in-depth comparative analyses.