Figure 2.

Overall process of neoantigen generation and presentation from tumor specific mutations.

Illustration demonstrating the key steps throughout the process of neoantigen generation, processing, binding, transportation and presentation. This overview highlights examples of criteria examined by pVACview that may be considered during neoantigen prioritization including: 1) founding clone versus subclonal tumor status of variants, 2) the impact of different transcripts structures on peptide sequence and their varying expression levels, 3) proteasomal processing generating peptides of varying length from different registers, 4) potential for peptide binding to individual patient MHC alleles and the predicted immunogenicity of peptide:MHC complexes, 5) variant and anchor positions of the neoantigen when presented to T-cells.