TABLE 2.
Approximation of growth ability of recombinant HPeV1 produced in monolayers of GMK cells
| Construct | Time when plaques were visible (days after transfection) | Relative virus growth in GMK cellsa |
|---|---|---|
| pHPeV1 | 3 | ++++++ |
| pHPeV1/1Δ168 | —b | −−−−−− |
| pHPeV1/86Δ168 | — | −−−−−− |
| pHPeV1/168Δ298 | — | −−−−−− |
| pHPeV1/298Δ538 | — | −−−−−− |
| pHPeV1/ΔD | 4 | +++−−− |
| pHPeV1/ΔH | 5 | ++−−−− |
| pHPeV1/ΔK | — | −−−−−− |
| pHPeV1/ΔL | 3 | ++++++ |
| pHPeV1/GNRA424 | —c | −−−−−− |
| pHPeV1/GNRA499 | 3 | ++++++ |
| pHPeV1/AUG710 | —c | −−−−−− |
| pHPeV1/AUG737 | 3 | ++++++ |
a
Ranging from ++++++ (wild-type growth) to −−−−−− (no growth), based on plaque size and period after transfection required for visible plaques to be observed.
b
—, no plaques or cytopathic effect visible after 6 days.
c
Revertants with wild-type sequence were obtained following blind passage.