FIG. 11.

TEM of HSV-1 viruses harboring the UL53 gene from 8MA-V. Vero cells were infected with KOS/UL53syn (A), 8MA-V (B and C), and 8MA/ΔSma/UL53syn (D) at an MOI of 0.1 and harvested 24 h postinfection. Alteration of gK results in the formation of both large syncytia and perinuclear “pockets” caused by fusion of adjacent outer nuclear membranes. 8MA-V and 8MA/ΔSma/UL53syn enveloped virions are blocked within the perinuclear space, often adjacent to areas of nuclear membrane fusion (thick arrows). In addition, full capsids are often viewed within the cytoplasm of infected cells (thin arrow). Bars, 0.5 μm.