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. 2000 Jul;74(14):6287–6299. doi: 10.1128/jvi.74.14.6287-6299.2000

FIG. 6.

FIG. 6

Southern blot analysis of 8MA-V DNA. Total DNA harvested from Vero cells infected with KOS, 8MA, or 8MA-V was cleaved with PstI and XhoI. The resulting Southern blot was hybridized with a 2.8-kb PstI/XhoI fragment spanning the VP16 locus. 8MA-V displayed the 3.7- and 1.9-kb VP16 fragments indicative of the 8MA VP16 deletion-lacZ substitution mutation.