TABLE 1.

Quantitative analysis of Vero cells infected with various strains of HSV-1

Parameter	Strain
	KOS	8MA/ΔSma	8MA
No. of cells counted	20	30	30
No. of cells with visible viral particles	19	19	12
No. of capsids/cell counted in:
Nucleus	55.6	8.3	2.2
Cytoplasm	6.1	0.9	0.2
No. of enveloped particles/cell counted in:
Perinuclear space	6.5	2.7	0.2
Cytoplasm	1.1	0	0
Extracellular space	23	0	0
Total no. of viral particles/cell counted	92.3	11.9	2.6
Total no. of viral particles relative to KOS (%)	100	13	3
% Packaged DNAa	100	31	4

^aAmount of packaged DNA relative to KOS as calculated from the total signal of samples treated with DNase (see Fig. 2, experiment 1).