Skip to main content
. Author manuscript; available in PMC: 2024 Jul 1.
Published in final edited form as: Nat Chem Biol. 2024 Jan 2;20(7):823–834. doi: 10.1038/s41589-023-01514-z

Extended Data Fig. 7. Extended orthogonal validation of functional sites.

Extended Data Fig. 7

(a) MTHFD2 probe 6 label sites overlapping with LY345899-binding site (PDB: 5TC4). (b) Immunoblot analysis of competitive blockade of probe 6-MTHFD2 interaction using LY345899. (c) ACAT2 probe 8 label sites overlapping with CoA-binding site (PDB: 1WL4). (d) Immunoblot analysis of competitive blockade of probe 8-ACAT2 interaction using CoA. (e) Immunoblot analysis of competitive blockade of probe 3-ABHD12 interaction using DO264 (see Supplementary Figure 12a for corresponding ABHD12 structure and probe 3 peptide plot). Each immunoblot displayed is representative of two independent experiments. (PD = pulldown)