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Analysis of cell-to-cell spread of recombinant viruses by plaque assay. MDCK cell monolayers were infected with wild-type viruses (WT) or recombinant viruses of the N1 NA and N2 NA groups as indicated. Monolayers were covered with an agarose-containing overlay for 3 days and stained with crystal violet. Dilutions were chosen individually for each virus stock in order to obtain optimal plaque pictures.
