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Release of recombinant viruses from MDCK cells. MDCK cells were infected at an MOI of 5 with recombinant viruses and incubated at 37°C overnight. One hour before virus harvest, VCNA was added to the culture medium of one half of the samples. Titers of progeny viruses released into the medium were determined by plaque assay. Levels of virus release in the absence of VCNA are presented as a percentage of the virus titers released after VCNA treatment.
