Fig. 2. Blind evaluation of virtual and true H& E stained lung cancer cores.

a Subjective quantification of true and virtual H& E staining of 12 TMA cores by three pathologists. b Overall quantification by the inter-observer scores of true and virtual histological images. Each score combines the evaluation of nuclear and cytoplasmic detail, overall staining quality, and diagnostic confidence based on staining quality, quantified using a range of 1 (unacceptable) to 4 (perfect).