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. 2024 Jun 28;15:5489. doi: 10.1038/s41467-024-49449-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic illustration of the experimental setup. b Unsupervised clustering of cells with microglial identity reveals three distinct clusters. c The three microglial clusters contain distinct proportions of tdTomato positive cells. d Quantification of tdTomato-PLIN2 positive and negative cells per cluster identifies cluster 2 as highly enriched for microglia containing LDs, while cluster 1 contains a majority of tdTomato negative cells. e The Plin2 expression matches this distribution, validating the sorting approach. f A heatmap of the top cluster specific genes shows a clear separation of the tdTomato-PLIN2 positive cluster 2, whereas cluster 1 and 0 had a less striking difference in gene expression. g Volcano plot of differentially expressed genes between cluster 2 and cluster 1. h A gene ontology (GO) analysis of the upregulated genes in cluster 2 reveals the enrichment of several terms involved in cellular activation as well as in immune functions.