Table 1.
Activities of UGTs toward phenolic compoundsa
| UGT | Activities (mU mg−1, N = 2) | |||||
|---|---|---|---|---|---|---|
| p-HAP | Phloretin | Apigenin | Luteolin | Daidzein | Activity with other sugar donorsc | |
| UGT84A49 |
31.4 ± 0.6 (4-O) |
/b |
34.2 ± 0.8 (7-O) 18.9 ± 0.4 (4′-O) 7.8 ± 0.8 (7,4′-di-O) |
14.7 ± 0.1 (7-O) 27.5 ± 0.1 (4′-O) 10.2 ± 0.6 (7,4′-di-O) |
224 ± 11.7 (7-O) |
38.5 ± 0.1 (Daidzein, UDP-Xyl) |
| UGT84A119 |
12.4 ± 0.3 (4-O) |
0.4 ± 1.4 × 10−3 (2′-O) 2.4 ± 1.4 × 10−2 (4′-O) |
64.7 ± 3.0 (7-O) 0.3 ± 2.8 × 10−2 (4′-O) 3.9 ± 0.2 (7,4′-di-O) |
83.1 ± 0.1 (7-O) 5.6 ± 0.3 (3′-O) 4.9 ± 0.4 (4′-O) 1.3 ± 7.0 × 10−2 (7,3′-di-O) 1.9 ± 0.1 (7,4′-di-O) |
171 ± 21.2 (7-O) |
9.6 ± 0.6 (Daidzein, UDP-Gal) 6.5 ± 0.4 (Daidzein, UDP-Xyl) |
| UGT72D1 |
171 ± 5.4 (4-O) |
34.7 ± 0.4 (2′-O) 4.3 ± 0.1 (4′-O) |
9.8 ± 0.6 (7-O) 4.5 × 10−2 ± 2.8 × 10−3 (7,4′-di-O) |
14.2 ± 0.6 (7-O) 22.3 ± 0.1 (3′-O) 7.5 × 10−2 ± 3.5 × 10−3 (7,3′-di-O) |
/b |
2.9 ± 0.1 (p-HAP, UDP-Xyl) |
| UGT72D7 |
2.4 ± 0.2 (4-O) |
/b | /b |
0.5 ± 4.5 × 10−3 (7-O) 7.2 ± 0.8 (4′-O) 0.1 ± 3.0 × 10−3 (7,4′-di-O) |
/b | /b |
aReactions (100 μL) contained 1.0 mM acceptor substrate, 2.0 mM UDP-sugar and 0.50 mg mL−1 enzyme in potassium phosphate buffer (50 mM, pH 8.0), and were carried out at 30 °C without agitation.
b“/” stands for no products detected in the reaction.
cUDP-Xyl, UDP-Gal and UDP-GlcA were tested. Daidzein was used as acceptor for UGT84A49 and UGT84A119, while p-HAP and luteolin were chosen for UGT72D1 and UGT72D7, respectively.