FIG. 2.
Southern hybridization analysis of the PrV parental, rescuant, and recombinant viral genomes. BamHI (A)- and BamHI-EcoRI (B)-restricted viral DNA fragments were separated by gel electrophoresis, transferred to a nylon membrane, and then hybridized sequentially with 32P-labeled DNA probes (Becker5′, Becker3′, LP, and lacZ) as indicated above each autoradiogram. The parental and rescue viral DNAs were resolved in lanes 1 and 2, respectively; genomes of two distinct recombinant M5′ isolates were resolved in lanes 3 and 4. Sizes of the viral DNA fragments complementary to the various probes are indicated on the right.