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. 2000 Jul;74(14):6333–6338. doi: 10.1128/jvi.74.14.6333-6338.2000

FIG. 5.

FIG. 5

Analysis of the PrV gD and E. coli lacZ gene PCR amplicons and the processed PrV LAT RT-PCR products generated from the porcine TG tissue preparations. Individual pigs are identified by number and represent uninfected negative controls (CC), parental virus-infected (Be), recombinant virus-infected (M5′), and rescuant-infected (R5′) animals. (A) Autoradiogram of gD gene amplicons using DNA probe gD. The positive control (Pos) uses PrV-Be DNA as the template and shows the anticipated 217-bp product. (B) Autoradiogram of LAT RT-PCR products using DNA probe LAT-MS. The positive control (Pos) uses pAC38 as the template and shows the predicted 252-bp product. (C) Electrophoregram of lacZ PCR products generated using primers A and C. The positive control (Pos) uses the lacZ insert in pRR12 as the template and shows the expected 327-bp product.