Table 4.

Rates of association of CKIIp with Ca²⁺-CaM in the presence of Ng

Ca2+ (μM)	Tyrosine fluorescence + Ng				Inter-FRET + Ng				Intra-FRET + Ng
	Rate 1 (s−1)	Amp 1	Rate 2 (s−1)	Amp 2	Rate 1 (s−1)	Amp 1	Rate 2 (s−1)	Amp 2	Rate (s−1)	Amp
0.26	0.5 ± 0.1	17	0.2 ± 0.1	54	0.7 ± 0.2	33	0.19 ± 0.1	56	0.5 ± 0.1	71
0.50	1.2 ± 0.1	41	0.4 ± 0.1	52	3.0 ± 0.1	25	0.68 ± 0.1	70	1.5 ± 0.1	86
1.10	4.7 ± 0.2	45	1.3 ± 0.1	56	9.6 ± 0.3	26	2.3 ± 0.1	72	4.5 ± 0.2	95
2.19	15.3 ± 0.5	61	4.3 ± 0.1	40	51 ± 2	22	9.0 ± 0.1	76	17.5 ± 0.5	100
3.26	30.9 ± 1.4	72	11.1 ± 0.2	28	128 ± 10	28	20.3 ± 0.2	74	32.9 ± 1.4	93
4.56	59.1 ± 5.8	57	21.7 ± 0.4	40	246 ± 18	30	33.3 ± 0.4	71	48.0 ± 1.9	94