Table 5.
Pseudo first-order calcium-dependent association rate constants
| Tyr |
Tyr + CKIIp |
Inter-FRET |
Intra-FRET |
|||
|---|---|---|---|---|---|---|
| k1.0 | k1.0R1 | k1.0R2 | k1.0R1 | k1.0R2 | k1.0 | |
| CaM | 5.4 | 10.6 | 5.2 | 84.2 | 8.0 | 15 |
| CaM +PEP-19 | 85.5 | 17.9 | 62.1 | 6.6 | 13.8 | |
| CaM +Ng | 6.4 | 1.8 | 22.5 | 4.6 | 3.8 | |
Rates (k) were derived by first fitting the data in Tables 1, 2, and 3 to linear or quadratic functions. The best fits were used to calculate the first derivative (tangent) at 1.0 μM Ca2+F, which is the apparent pseudo first-order rate constant at that level of Ca2+F.
Numbers in bold indicate rates associated with the dominant amplitude.