Figure 4.

Putative lipid binding sites from coarse-grained MD trajectories. The lipids are color coded as follows: CHOL in red, PIP2 in magenta, POPC in blue, POSM in orange. The position of the backbone atoms within 0.6 nm of the bound lipids are shown as cyan spheres. Since NDCBE and AE1_OF-OF did not show enrichment of the POSM around the protein matrix or long-lived protein-POSM interactions, we have not identified POSM binding sites in these two systems. (Inset) Designation of the core and gate domains of monomers A and B of the SLC4 dimers and TM numbering, using AE1_OF-OF as an example. (Top) Surface representation of AE1 divided into gate (blue) and core (white) domains; (bottom) helical representation of AE1 with individually colored TMs. To see this figure in color, go online.