Figure 5.

Sequence alignment of the four studied SLC4 systems. The identified putative lipid sites in Fig. 4 are highlighted in different colors, depending on the lipid type. The binding sites are formulated as backbone residues within 0.6 nm of the bound lipids (cyan sphere in Fig. 4). The lipid binding sites are color coded as in Fig. 4 (CHOL in red, PIP2 in magenta, POPC in blue, POSM in orange) and presence of more than one color at an amino acid residue signifies participation of this residue in more than one binding site. The CRAC/CARC domains in the areas of putative CHOL sites are displayed as gray boxes. See Fig. 4 for designation of the core/gate domains and TM numbering of the SLC4 monomers. To see this figure in color, go online.